A Comparison of Different Particle Deposition Methods on Dissolution using Transwell^® Setup

Abhimata Paramanandana¹, Magda Swedrowska¹ & Ben Forbes¹

¹ Institute of Pharmaceutical Science, King’s College London, Waterloo Road, London, SE1 8WA, United Kingdom

Summary

The study compared aerosolized particles collected using different deposition techniques by investigating the dissolution of Budesonide delivered by 400 µg Novolizer^®. Drug was aerosolized using a flow of 60 L/min into an abbreviated and modified Andersen cascade impactor system and collected onto a GF/A glass microfiber using methods which favoured impaction or sedimentation. In the sedimentation method, the collection filters are placed in two different configuration levels: at the bottom of the hollow stage (Sedimentation-1) and the end filter stage (Sedimentation-2). A burst of airflow (0.4 – 0.7 s) to carry particles into the hollow chamber was performed followed by a sedimentation time of 20 min between actuations. The dissolution setup was a 6-well Transwell^® system agitated at 60 rpm using an incubator shaker maintained at 37°C. Dissolution medium was 0.1% SDS in PBS with 2.35 mL and 0.1 mL as receptor and donor chamber volumes, respectively. Particle mass collected was between 25.6 – 28.3 µg, 6 – 12.5 µg and 37.2 – 48.5 µg for Impaction, Sedimentation-1, and Sedimentation-2, respectively. Scanning electron micrograph showed several aggregates were observed in , Sedimentation-1 whereas denser particles were observed both in Impaction and Sedimentation-2. Dissolution of all particle collection methods did not show significant differences.